Quantitative Determination of Coenzyme A by Sorbyl Coenzyme A Formation

Formation of lactyl-coenzyme A and pyruvyl-coenzyme A from lactic acid by Escherichia coli.

Megraw, Robert E. (Albert Einstein Medical Center, Philadelphia, Pa.), Henry C. Reeves, and Samuel J. Ajl. Formation of lactyl-coenzyme A and pyruvyl-coenzyme A from lactic acid by Escherichia coli. J. Bacteriol. 90:984-988. 1965.-Cell extracts of propionate-adapted Escherichia coli were found to contain a lactyl-coenzyme A (CoA) synthetase which catalyzes the formation of the CoA thiolester fr...

Determination of Coenzyme A and Acetyl-Coenzyme A in Biological Samples Using HPLC with UV Detection.

Coenzyme A (CoA) and acetyl-coenzyme A (acetyl-CoA) play essential roles in cell energy metabolism. Dysregulation of the biosynthesis and functioning of both compounds may contribute to various pathological conditions. We describe here a simple and sensitive HPLC-UV based method for simultaneous determination of CoA and acetyl-CoA in a variety of biological samples, including cells in culture, ...

Regulation of the activity of acetyl coenzyme A carboxylase by palmitoyl coenzyme A and citrate.

Pahnitoyl-CoA (100 PM), in the presence of albumin (24 mg per ml), inhibited the incorporation of [14C]citrate into fatty acids in a cytosol fraction of chick liver and inhibited the activity of acetyl-CoA carboxylase purified from chick liver. Under similar incubation conditions, pahnitoyl-CoA (ZOO PM) did not inhibit fatty acid synthetase, ATP-citrate lyase, malic enzyme, NADP-linked isocitra...

Quantitative determination of coenzyme Q10 from dietary supplements by FT-NIR spectroscopy and statistical analysis.

A novel, time- and money-sparing method has been developed and validated for the quantitative determination of coenzyme Q10 (CoQ10) from several dietary supplements. FT-NIR spectroscopy was applied for the examination, and a calibration model was built by partial least-square regression (PLS-R) using 50 dietary supplements. The combination of FT-NIRS and multivariate calibration methods is a ve...

Utilization of coenzyme A by Propionibacterium freudenreichii.